热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 蛋白 细胞因子

R-spondin 1 Protein, Mouse

RSPO1,R-spondin1,RP11-566C13.1,CRISTIN3,FLJ40906,RSPO Rspo1,R-spondin,Rspondin,RP23-325M14.2,Roof plate-specific spondin-1

价格 ￥680.00 （ 1-2周）
货号 UA100019
规格
数量
收藏 分享
产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Mouse

  • 分子别名

    RSPO1; R-spondin1; RP11-566C13.1; CRISTIN3; FLJ40906; RSPO Rspo1; R-spondin; Rspondin; RP23-325M14.2; Roof plate-specific spondin-1

  • Accession

    Q9Z132

  • 表达序列

    Ser21-Gly209

  • 表达宿主

    E.coli

  • 分子量

    Approximately 20.9 kDa, a single non-glycosylated polypeptide chain containing 189 amino acids.

  • 纯度

    ＞96% by SDS-PAGE or HPLC.

  • 活性

    Fully biologically active when compared to standard. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED₅₀ is 50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized powder

  • 缓冲体系

    20 mM PB, with 150 mM NaCl, pH 7.4.

  • 溶解方法

    Before use this product, please read the direction below carefully.
    This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
    Reconstitute in a sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.
    Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
    Further dilutions should be made in appropriate buffered solutions.

  • 储存条件

    For long term storage, the product should be stored ≤ -20℃.
    Please avoid repeated freeze-thaw cycles after reconstitution.
    12 months from date of receipt, -20 to -70℃ as supplied.
    1 month, 2 to 8℃ under sterile conditions after reconstitution.
    3 months, -20 to -70℃ under sterile conditions after reconstitution.

背景介绍

  • R-Spondin 1, also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3) , is a 27kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members (1, 2) . All R-Spondins regulate Wnt/beta-Catenin signaling but have distinct expression patterns. R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization. However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly. R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development. I Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9) . Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis. Mouse R-Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively, within aa 21-209. Recombinant Mouse R-Spondin 1 is a single non-glycosylated polypeptide chain containing 189 amino acids

评论(0)