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产品中心 蛋白 细胞因子

IL-10 Protein, Mouse

Interleukin-10,CSIF

价格 ￥1,030.00 （现货 ： 2-5个工作日）
货号 UA100018
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Mouse

  • 分子别名

    Interleukin-10, CSIF

  • Accession

    P18893

  • 表达序列

    Ser19-Ser178

  • 表达宿主

    E.coli

  • 分子量

    Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 160 amino acids.

  • 纯度

    ＞96% by SDS-PAGE or HPLC.

  • 活性

    Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of ≥ 1.0×10⁶ IU/mg.

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    20 mM PB, with 150 mM NaCl, pH 7.4.

  • 溶解方法

    Before use this product, please read the direction below carefully.
    This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
    Reconstitute in 10 mM HCl to a concentration of 0.1-1.0 mg/ml.
    Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
    Further dilutions should be made in appropriate buffered solutions.

  • 储存条件

    For long term storage, the product should be stored ≤ -20℃.
    Please avoid repeated freeze-thaw cycles after reconstitution.
    12 months from date of receipt, -20 to -70℃ as supplied.
    1 month, 2 to 8℃ under sterile conditions after reconstitution.
    3 months, -20 to -70℃ under sterile conditions after reconstitution.

背景介绍

  • Interleukin-10 (IL-10) is encoded by the IL10 gene and expressed in monocytes and Type 2 T helper cells (Th2) . The biological activities of IL-10 are mediated by the IL-10 receptor complex, which is composed of the ligand-binding IL-10 Rα/R1 and the accessory IL-10 Rβ/R2 subunits. IL-10 is capable of inhibiting the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by T helper cells. IL-10 is classified as a class 2 cytokine which consist of IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26, several interferons and interferon-like molecules. Mature murine IL-10 shares 70%-77% amino acid sequence identity with human, bovine, porcine IL-10, but murine IL-10 does not act on human cells. Recombinant murine IL-10 is an 18.7kDa protein containing 160 amino acid residues.

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