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物种
Human分子别名
T-lymphocyte differentiation antigen T8/Leu-2, MALAccession
P01732表达序列
Ser22-Asp182, with C-hFc
表达宿主
HEK293分子量
Predicted MW: 43.7 kDa Observed MW: 55 kDa
纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
Human Fc Tag性状
Lyophilized Powder缓冲体系
0.2M PBS, pH7.4.
溶解方法
Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
储存条件
12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
CD8 (cluster of differentiation 8) is a transmembrane glycoprotein that serves as a co-receptor for the T-cell receptor (TCR). Along with the TCR, the CD8 co-receptor plays a role in T cell signaling and aiding with cytotoxic T cell-antigen interactions. Like the TCR, CD8 binds to a major histocompatibility complex (MHC) molecule, but is specific for the MHC class I protein. There are two isoforms of the protein, alpha and beta. The extracellular IgV-like domain of CD8-α interacts with the α3 portion of the Class I MHC molecule. Cytotoxic T cells with CD8 surface protein are called CD8+ T cells. Once the T cell receptor binds its specific antigen Lck phosphorylates the cytoplasmic CD3 and ζ-chains of the TCR complex which initiates a cascade of phosphorylation eventually leading to activation of transcription factors like NFAT, NF-κB, and AP-1 which affect the expression of certain genes.
电泳
2μg（R: reducing conditions）
ELISA
Immobilized Human CD8, hFc tag at 1 μg/mL (50 μL/well) can bind CD8α Mouse mAb (SDT-1036-34) (Cat. No. S0B2307) with EC50 of 15.68-23.01 ng/ml.
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