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物种
Human分子别名
Interleukin-9;IL-9;Cytokine P40;T-cell growth factor P40;IL9Accession
P15248表达序列
Gln19-Ile144
表达宿主
E.coli分子量
Approximately 14.1 kDa, a single non-glycosylated polypeptide chain containing 126 amino acids.
纯度
＞97% by SDS-PAGE or HPLC.活性
Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human MO7e cells is less than 0.2 ng/ml, corresponding to a specific activity of ≥ 5.0×10⁶ IU/mg.标记
Unconjugated性状
Lyophilized Powder缓冲体系
20 mM PB, with 150 mM NaCl, pH 7.4.
溶解方法
Before use this product, please read the direction below carefully.
This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
储存条件
For long term storage, the product should be stored ≤ -20℃.
Please avoid repeated freeze-thaw cycles after reconstitution.
12 months from date of receipt, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Interleukin-9 (IL-9) is encoded by the IL9 gene and produced by T-cells and specifically by CD4+ helper cells. IL-9 is an immunoregulatory cytokine enhances the proliferation of T lymphocytes, mast cells, erythroid precursor cells and megakaryoblastic leukemia cell lines. Over-expression of IL-9 has been implicated in the pathogenesis of anaplastic lymphoma and Hodgkin’s disease. Whereas murine IL-9 can function on human cells, human IL-9 is inactive on mouse cells. Recombinant Human IL-9 is a 14.1kDa protein of 126 amino acid, including 10 cysteine that are fully conserved between the human and murine proteins.
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