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5x激酶缓冲液A

价格 150.00 供应商现货 : 3-5个工作日
货号 UA085054
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产品规格
  • 性状

    Liquid
  • 储存条件

    For long term storage, keep 5X buffer at -20°C. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for 1-2 weeks.

背景介绍
  • Kise Buffer can be used to assay protein kise activity.

产品组分
  • 200 mM Tris-HCl, pH 7.4, 100 mM MgCl2, and 0.5 mg/mL BSA. Add fresh DTT prior to use to a fil concentration of 250 μM.

操作步骤
  • 1. Thaw 5X buffer at 24-30°C, mixing end-over-end.

    2. Dilute the 5X buffer to the desired concentration using ddH2O.

注意事项
  • For optimal storage, aliquot buffer into smaller quantities after centrifugation and store at recommended temperature. For most favorable

    performance, avoid repeated handling and multiple freeze/thaw cycles.

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