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产品中心 药物靶点和Fc受体 小分子药物靶点蛋白

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IDH1(R132C) His Tag Protein, Human

IDH1,PICD,IDP

价格 ￥500.00 （ 供应商现货 ： 3-5个工作日）
货号 UA085027
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    IDH1, PICD, IDP

  • Accession

    O75874-1

  • 表达序列

    Met 1-Leu 414 with His Tag at the C-Terminus

  • 表达宿主

    E.coli

  • 分子量

    40-55kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE & HPLC

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Liquid

  • 缓冲体系

    50mM Tris, 150mM NaCl, pH7.5, 1mM DTT, 10%Glycerol

  • 储存条件

    Stable for 12 months upon stored at -80℃ from the date of receipt.
    And avoid repeated freeze-thaws cycles.

  • 文献引用

    1. Parsons, D.W., et al. (2008). An integrated genomic analysis of human glioblastoma multiforme. Science, 321(5897), 1807-1812.
    2. Dang, L., et al. (2009). Cancer-associated IDH1 mutations produce 2-hydroxyglutarate. Nature, 462(7274), 739-744.
    3. Xu, W., et al. (2011). Oncometabolite 2-hydroxyglutarate is a competitive inhibitor of α-ketoglutarate-dependent dioxygenases. Cancer Cell, 19(1), 17-30.

背景介绍

  • IDH1 (Isocitrate Dehydrogenase 1) is a key metabolic enzyme located in the cytoplasm and peroxisomes. Its primary physiological function is to catalyze the oxidative decarboxylation of isocitrate to produce alpha-ketoglutarate (α-KG), while concurrently reducing NADP+ to NADPH. This reaction is crucial for cellular metabolism, as it links the tricarboxylic acid (TCA) cycle with lipid metabolism and provides NADPH, a major reducing agent essential for antioxidant defense and anabolic biosynthesis.

    A seminal discovery in cancer biology revealed that specific heterozygous somatic mutations in the IDH1 gene, most commonly at the R132 residue, are frequent drivers in several cancers. These include gliomas (especially secondary glioblastoma and oligodendroglioma), acute myeloid leukemia (AML), cholangiocarcinoma, and chondrosarcomas. The mutant enzyme acquires a neomorphic activity: instead of producing α-KG, it reduces α-KG to D-2-hydroxyglutarate (D-2HG). This oncometabolite, D-2HG, accumulates to high levels and functions as a competitive inhibitor of multiple α-KG-dependent dioxygenases. This inhibition leads to profound epigenetic dysregulation (via inhibition of histone and DNA demethylases) and blockade of cellular differentiation, thereby promoting tumorigenesis.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • The purity of IDH1(R132C) His Tag Protein, Human is more than 95% determined by SEC-HPLC.

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