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物种
Human分子别名
CHIP, PP1131, STUB1, E3 ubiquitin-protein ligase CHIP, Antigen NY-CO-7Accession
Q9UNE7表达序列
Ser23-Tyr303
表达宿主
E.coli分子量
33-43kDa (Reducing)
纯度
＞95% by SDS-PAGE & HPLC标记
Unconjugated标签
No Tag性状
Liquid缓冲体系
50mM Tris, 150mM NaCl, PH7.5, 1mM DTT, 10%Glycerol
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
文献引用
1. Ballinger, C.A., et al. (1999). Identification of CHIP, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions. Molecular and Cellular Biology, 19(6), 4535-4545.
2. Jiang, J., et al. (2001). CHIP is a U-box-dependent E3 ubiquitin ligase: identification of Hsc70 as a target for ubiquitylation. Journal of Biological Chemistry, 276(46), 42938-42944.
STUB1 (STIP1 Homology and U-Box Containing Protein 1), commonly known as CHIP (C-terminus of Hsc70 Interacting Protein), is a crucial dual-function protein in cellular proteostasis. It serves as both a co-chaperone and an E3 ubiquitin ligase. As a co-chaperone, it binds to the molecular chaperones Hsp70/Hsc70 and Hsp90 via its N-terminal tetratricopeptide repeat (TPR) domain, assisting in client protein folding and stabilization. Its central role lies in its C-terminal U-box domain, which confers E3 ubiquitin ligase activity. This unique combination allows CHIP to ubiquitinate misfolded or damaged client proteins that are delivered by the chaperones, thereby targeting them for proteasomal degradation. It is a central component of the Protein Quality Control (PQC) system, clearing toxic protein aggregates and maintaining proteome integrity.
Dysregulation of CHIP function is linked to various human diseases. Loss-of-function mutations or reduced expression are associated with neurodegenerative disorders (e.g., spinocerebellar ataxia, Alzheimer's, Parkinson's) due to defective clearance of pathogenic proteins like tau and α-synuclein. Conversely, its role in degrading oncogenic clients (e.g., HER2, c-Myc) positions it as a potential tumor suppressor in certain cancers. It also plays key roles in aging, cardiac function, and immune responses, making it a significant therapeutic target.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of STUP1/CHIP Protein, Human is more than 95% determined by SEC-HPLC.
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