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产品中心 药物靶点和Fc受体 小分子药物靶点蛋白

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SKP2 GST Tag Protein, Human

FBXL1

价格 ￥2,200.00 （ 供应商现货 ： 3-5个工作日）
货号 UA085002
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    FBXL1

  • Accession

    Q13309

  • 表达序列

    Arg89-Leu424 with GST Tag at the N-Terminus

  • 表达宿主

    E.coli

  • 分子量

    63kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 标记

    Unconjugated

  • 标签

    GST Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, PH7.4, 5% trehalose

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Schulman BA, Carrano AC, Jeffrey PD, et al. Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex. Nature. 2000;408(6810):381-386.

背景介绍

  • SKP2 (S-phase kinase-associated protein 2) is an F-box-containing substrate-recognition subunit of the SCFSKP2 E3 ubiquitin ligase complex (Cullin-1-SKP1-SKP2-Rbx1). Its N-terminal F-box docks onto SKP1, while C-terminal leucine-rich repeats (LRRs) capture phosphorylated targets. By catalysing K48-linked poly-ubiquitylation, SKP2 drives proteasomal degradation of a panel of tumour suppressors and cell-cycle brakes, including p27Kip1, p21Cip1, p57Kip2, FOXO1, BRCA2 and RASSF1A, thereby promoting G1/S progression, DNA replication and survival signalling.

  • 电泳

    • 2μg (R: reducing condition).

  • ELISA

    • Immobilized SKP2 GST Tag Protein, Human (Cat. No. UA085002) at 2.0μg/mL (100μL/well) can bind Biotinylated CKS1 GST Tag Protein, Human(Cat. No.UA085010) with EC50 of 45.26-63.21 ng/mL.

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