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产品规格
物种
Human分子别名
Cyclin-dependent kinase 9/CyclinT1Accession
CDK9: P50750, CyclinT1: O60563-1表达序列
CDK9: M1-F372(end), N-terminal GST tag;
CyclinT1: M1-K726(end), N-terminal GST tag, C-terminal 8x His tag表达宿主
Baculovirus-InsectCells分子量
CDK9: 68.6 kDa; Cyclin T1: 107.8 kDa纯度
> 75% by SDS-PAGE标记
Unconjugated标签
GST Tag, His Tag性状
Liquid缓冲体系
20 mM Tris, 300 mM NaCl, 20 mM GSH, 10% glycerol, pH7.5
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The CDK9/CyclinT1 protein was incubated with ATP and substrate at 25 °C for 60 minutes. Following the incubation, ADP-Glo reagent was added to the reaction mixture. The luminescent signal (RLU) was finally measured using a BMG microplate reader.
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