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产品规格
物种
Human分子别名
PIK3CA,CLAPO,CLOVE,CWS5,MCAP,MCM,MCMTC,PI3K,PI3K-alpha,p110-alphaAccession
PIK3CA: P42336, PIK3R1: P27986-1表达序列
PIK3CA: M1-N1068(end); N-terminal GST tag
PIK3R1: M1-R724(end); N-terminal Flag tag表达宿主
Baculovirus-InsectCells分子量
PIK3CA: 150.9 kDa; PIK3R1: 84.9 kDa纯度
> 80% by SDS-PAGE标记
Unconjugated标签
Flag Tag, GST Tag性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The PIK3CA/PIK3R1 activity was activity was quantified using the ADP-Glo assay. The reaction was performed by incubating the PIK3CA/PIK3R1 protein, ATP and substrate at 25℃ for 60 min, adding ADP-Glo reagent and then measuring luminescence signal on BMG.
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