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产品规格
物种
Human分子别名
EMT, LYKAccession
Q08881表达序列
N2-L620(end); N-terminal GST tag
表达宿主
Baculovirus-InsectCells分子量
97.5 kDa纯度
> 85% by SDS-PAGE标记
Unconjugated标签
GST Tag性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% glycerol, pH7.5
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The ITK activity was detected using HTRF technology. The reaction was performed by incubating the ITK protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
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