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产品规格
物种
HumanAccession
P24941-1, P24864-1表达序列
CDK2: M1-L298(end);
CyclinE1: M1-A410(end)表达宿主
Baculovirus-InsectCells分子量
CDK2: 34.1 kDa; CyclinE1: 47.2 kDa纯度
> 95% by SDS-PAGE标记
Unconjugated标签
No Tag性状
Liquid缓冲体系
20 mM Tris, 150 mM NaCl, 1 mM DTT, pH7.5
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The CDK2/CyclinE1 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the CDK2/CyclinE1 protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
电泳
体积排阻色谱(SEC-HPLC)







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