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产品中心 药物靶点和Fc受体 小分子药物靶点蛋白

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DCAF15/DDB1 His&StrepII Tag Protein, Human

价格 ￥2,980.00 （ 1-2周）
货号 UA080523
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • Accession

    Q66K64, Q16531

  • 表达序列

    A2-L600 end (DCAF15), N-His-StrepII-TEV-GG
    M1-H1140 end (DDB1), No tag

  • 表达宿主

    Baculovirus-InsectCells

  • 分子量

    69.6 KDa/126.9 KDa

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Liquid

  • 缓冲体系

    50 mM Tris-HCl (pH 7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT

  • 储存条件

    -80℃

注意事项
  • Please use rapid thawing with running water to thaw the protein samples.
    For complete recovery, mix well and spin before use.
    Product must not be stored in diluted solutions.
    Aliquots below 10μl are not advisable.
    Avoid repetitive freeze-thaw cycles!

  • 生物活性

    • Gel based ligase assay
      Lane M: Marker
      Lane 1: Bodipy-Ub + UBE1 + Ubch5b + DCAF15/DDB1 with ATP
      Lane 2: Bodipy-Ub + UBE1+ Ubch5b with ATP
      Lane 3: Bodipy-Ub + UBE1+ Ubch5b without ATP
      The ubiquitin conjugating activity of DCAF15/DDB1 was validated through its ability to catalyse
      the generation of polyubiquitin chains in the presence of the E1 activating enzyme UBE1, the E2
      conjugating enzyme Ubch5b and Bodipy-ubiquitin. Incubation of DCAF15/DDB1 for 60 minutes at
      37˚C in the presence of Bodipy-ubiquitin, UBE1, Ubch5b and ATP (Lane 1) was compared
      alongside two control reactions with either DCAF15/DDB1 (Lane 2) or DCAF15/DDB1&ATP
      (Lane 3) excluded from the reaction. Ubiquitin conjugates were identified by the migration of the
      Bodipy-ubiquitin band and these were observed only in the presence of ATP and DCAF15/DDB1.

  • 电泳

    • Lane M: Marker
      Lane 1: 2 μg, reduced
      Lane 2: 2 μg, non-reduced

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