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产品规格
物种
HumanAccession
P01116-2表达序列
M1-K169;N-terminal 8x His tag, C-terminal Avi tag
表达宿主
E.coli分子量
23.5 kDa
纯度
> 95% by SDS-PAGE标记
Unconjugated标签
Avi Tag, His Tag性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The Biotin-KRAS[Q61L] activity was detected using HTRF technology. The reaction was performed by incubating the Biotin-KRAS[Q61L] protein and substrate, adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 665/620 with BMG.
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