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产品规格
物种
MouseAccession
Q8C7D2-1, Q3U1J4表达序列
CRBN:M1-L445(end); N-terminal 8x His tag
DDB1: M1-H1140(end);no tag表达宿主
Baculovirus-InsectCells分子量
CRBN: 53.3 kDa; DDB1: 126.9 kDa
纯度
> 70% by SDS-PAGE标记
Unconjugated标签
No Tag, His Tag性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The CRBN/DDB1 activity was detected using TR-FRET technology. The reaction was performed by incubating the CRBN/DDB1 protein and Thalidomide-BODIPY, adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 520/490 with BMG.
电泳
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