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产品规格
物种
Human分子别名
SAK,STK18Accession
O00444表达序列
A2-H970(end) ; N-terminal GST tag-TEV, C-terminal Flag tag
表达宿主
Baculovirus-InsectCells分子量
136.6 kDa
纯度
>70% by SDS-PAGE标记
Unconjugated标签
GST Tag, Flag Tag性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 5 mM GSH, 1 mM DTT, 0.05% Brij35,10% glycerol, pH7.5
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The PLK4 kinase activity was quantified using the ADP-Glo assay. The reaction was performed by incubating the PLK4 protein, ATP and substrate at 25℃ for 60 min, adding ADP-Glo reagent and then measuring luminescence signal on BMG.
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