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产品规格
物种
HumanAccession
Q53GL7表达序列
G805-T1025 (end); N-terminal Flag tag, C-terminal Strep II tag
表达宿主
Baculovirus-InsectCells分子量
27.1 kDa
纯度
>95% by SDS-PAGE标记
Unconjugated标签
Flag Tag, Strep II tag性状
Liquid缓冲体系
20 mM Hepes, 300 mM NaCl, 1 mM DTT, pH7.0
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The PARP10 activity was assayed with ELISA. The reaction was performed by: 1). Histone protein was coated on a 96-well plate. 2).A biotinylated NAD+ mix is incubated with varying different concentration of the PARP10 enzyme and an activated DNA. 3).The plate was treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence. Finally, measured the signal using a chemiluminescence reader.
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