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产品规格
物种
HumanAccession
Q53GL7表达序列
G805-V1007; N-terminal Flag tag, C-terminal 6x His tag
表达宿主
Baculovirus-InsectCells分子量
25.0 kDa纯度
>95% by SDS-PAGE标记
Unconjugated标签
Flag Tag, His Tag性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% Glycerol, pH7.5
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The PARP10 activity was assayed with ELISA. The reaction was performed by: 1). Histone protein was coated on a 96-well plate. 2).A biotinylated NAD+ mix is incubated with varying different concentration of the PARP10 enzyme and an activated DNA. 3).The plate was treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence. Finally, measured the signal using a chemiluminescence reader.
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