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产品规格
物种
Human分子别名
PLKAccession
P53350表达序列
M1-S603(end); N-terminal GST tag, C-terminal 6x His tag
表达宿主
Baculovirus-InsectCells分子量
95.9 kDa纯度
>70% by SDS-PAGE标记
Unconjugated标签
GST Tag, His Tag性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% Glycerol, pH7.5
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The PLK1 activity was detected using Ultra Luminescence technology. The reaction was performed by
incubating the PLK1 protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading
RLU signal with BMG.
电泳
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