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产品规格
Accession
A0A8C0RYL7, A0A8C0QFD6表达序列
CRBN: M1-L444(end); N-terminal 8x His tagDDB1: M1-H1140(end); No tag表达宿主
Baculovirus-InsectCells分子量
CRBN: 53.4 kDa; DDB1: 127.0 kDa纯度
>90% by SDS-PAGE标记
Unconjugated标签
His Tag性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
背景介绍
Recombinant dog CRBN with N-terminal 8x His tag was co-expressed with recombinant dog DDB1 protein. Recombinant dog CRBN/DDB1 protein with N-terminal 8x His tag was purified by Ni-NTA affinity and followed by desalting. The CRBN/DDB1 protein showed high activity in TR-FRET assay.
生物活性
The CRBN/DDB1 activity was detected using TR-FRET technology. The reaction was performed by incubating the CRBN/DDB1 protein and Thalidomide-BODIPY, adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 520/490 with BMG.
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