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产品规格
物种
HumanAccession
O14744-1, Q9BQA1-1表达序列
PRMT5: A2-L637(end); N-terminal Flag and Avi tag
MEP50: 2R-E342(end); N-terminal 6x His tag表达宿主
Baculovirus-InsectCells分子量
PRMT5: 75.6 kDa; MEP50: 37.8 kDa纯度
>95% by SDS-PAGE标记
Unconjugated标签
Flag Tag, Avi Tag, His Tag性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% Glycerol, pH7.5
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The Biotin-PRMT5/MEP50 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the Biotin-PRMT5/MEP50 protein, SAM and substrate at 25℃ for 180 min, then adding MTase-Glo reagent and reading RFU with BMG.
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