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产品规格
物种
Human分子别名
BAF190A,BRG1,SNF2B,SNF2L4Accession
P51532-2表达序列
E658-E1361,with N-terminal 6x His-TEV-Avi tag
表达宿主
Baculovirus-InsectCells分子量
85.5 kDa纯度
>90% by SDS-PAGE标记
Unconjugated标签
Avi Tag, His Tag性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% glycerol, pH7.5
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The Biotin-BRG1 activity was detected using SPR technology. The Biotin-BRG1 protein was immobilized onto the SA chip coupled with neutravidin using labeled biotin. The varying concentration of Alpelisib was injected onto the Biotin- BRG1 protein surface and recorded the response signal. The kinetics fitting was as follows with a kD value of ~
5.96x10-9 M.
电泳
体积排阻色谱(SEC-HPLC)







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