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产品规格
物种
Human分子别名
GTPase KRas,KRAS2,RASK2Accession
P01116-2表达序列
M1-K169,with N-terminal 8x His tag-TEV, C-terminal Avi
表达宿主
E.coli分子量
23.6 kDa
纯度
>90% by SDS-PAGE标记
Unconjugated标签
Avi Tag, His Tag性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 10% glycerol, pH 7.5
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The Biotin-KRAS[G12C] activity was quantified using a HTRF-based assay. The reaction was performed by incubating the Biotin-KRAS[G12C] protein and substrate, the donor and receptor at 25℃ for 60 min, then measuring the 665nm/615nm fluorescence signal ratio on BMG.
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