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产品规格
物种
Human分子别名
GTPase KRas, KRAS2, RASK2Accession
P01116-2表达序列
M1-K169,with N-terminal 8x His tag,C-terminal Avi
表达宿主
E.coli分子量
23.6 kDa
纯度
>85% by SDS-PAGE标记
Unconjugated标签
Avi Tag, His Tag性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性JSON
The Kras[G12C](GDP Loaded) activity was detected using HTRF technology. The reaction was performed by incubating the Kras[G12C](GDP Loaded) protein, GTP and SOS1 at 25℃ for 60 min, then adding cRAF at 25℃ for 15 min and adding the beads at 25℃ for 60 min, reading ratio 665/620 with BMG.
电泳JSON
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