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产品规格
物种
HumanAccession
P17252表达序列
A2-V672 end,with N-GST-TEV
表达宿主
Baculovirus-InsectCells分子量
103.2 KDa
标记
Unconjugated标签
GST Tag性状
Liquid缓冲体系
50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 5% glycerol, 5 mM DTT, 0.1 M Trehalose
储存条件
-80℃
注意事项
Please use rapid thawing with running water to thaw the protein samples.
For complete recovery, mix well and spin before use.
Product must not be stored in diluted solutions.
Aliquots below 10μl are not advisable.
Avoid repetitive freeze-thaw cycles!
生物活性
The activity of PKCα is verified by the ADP-Glo method: Incubate PKCα protein, ATP, and the
substrate at room temperature for 40 minutes, then add the ADP-Glo reagent and read the
luminescence signal using the chemiluminescence module of the microplate reader.
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