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物种
HumanAccession
P27361表达序列
A2-P379 end, N-GST-TEV
表达宿主
E.coli分子量
69.3 KDa
纯度
＞85% by SGS-PAGE标记
Unconjugated标签
GST Tag性状
Liquid缓冲体系
50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT
储存条件
-80℃
Please use rapid thawing with running water to thaw the protein samples.
For complete recovery, mix well and spin before use.
Product must not be stored in diluted solutions.
Aliquots below 10μl are not advisable.
Avoid repetitive freeze-thaw cycles!
生物活性
The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with
fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were
separated and detected by MSA device. The conversion rate of ERK1 is 30% when it reacts with 20 μM ATP
and 2 μM Peptide 8 for 30 minutes, and the corresponding enzyme concentration is 25 nM.
电泳
Lane M: Marker
Lane 1: 2 μg, reduced
Lane 2: 2 μg, non-reduced
引用文献(1)
- Discovery of 2-Amino-7-Amide Quinazoline Derivatives as Potent and Orally Bioavailable Inhibitors Targeting Extracellular Signal-Regulated Kinase 1/2
Peili Zhang; Yu Zhang; Yuping Sun; Yanyan Chen; Xiang Cao; et al
Journal of Medicinal Chemistry. 2025 May 15 ; 0022-2623,1520-4804
影响因子： 6.8
货号：UA080437产品名称：ERK1 Protein, Human
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