热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 药物靶点和Fc受体 小分子药物靶点蛋白

所有图片（1/5）

Cullin2-RBX1-ElonginB/C-VHL Protein

VHL/ELOB/ELOC/CUL2/RBX1 Complex

价格 ￥2,200.00 （ 1-2周）
货号 UA080274
规格
数量
收藏 分享
产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    VHL/ELOB/ELOC/CUL2/RBX1 Complex

  • 表达序列

    His-RBX1 (full-length), HA-Cullin2 (full-length), HA-ElonginC (full-length), ElonginB (full-length), VHL (full-length)

  • 表达宿主

    Sf9 and E. coli

  • 纯度

    ＞90% by SDS-PAGE

  • 内毒素含量

    <1EU/μg

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    20mM HEPES, 200mM NaCl, 5% Glycerol, 2mM TCEP, pH 8.0

  • 储存条件

    Store at -80ºC. Avoid multiple freeze-thaw cycles.

背景介绍

  • VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues. Targets of VHL include HIF1 alpha, beta 2 adrenergic receptor, ZHX2 and others. In VHL-box E3 Ubiquitin ligases, VHL is bound to a heterodimer complex composed of Elongin B (ELOB) and Elongin C (ELOC) through the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families. ELOB/ELOC serves as an adapter between substrate recognition proteins and Cullin-2 (or Cullin-5) and RBX1 to form an active E3 ligase.

  • 生物活性

    • The Cullin2-RBX1-ElonginB/C-VHL activity was measured by protein-tracer binary binding assay using HTRF technology.

    • The Cullin2-RBX1-ElonginB/C-VHL activity was measured by PROTAC-mediated Ternary Complex Formation using HTRF technology. The dose response curve of ARV-771 in the Ternary Assay exhibited a bell-shaped curve, a hook effect and a peak.

    • The Cullin2-RBX1-ElonginB/C-VHL activity was measured by WB analysis for target protein ubiquitination assay. The Ubiquitinated BRD4(D1+D2) bands increased with time.

  • 电泳

    • Lane M: Precision Plus Protein™ Dual Color Standards (Bio-Rad) (Sample loaded: 5 μl)

      Lane 1: Cullin2-RBX1-ElonginB/C-VHL complex under reducing condition (Sample loaded: 2 μg)

  • 体积排阻色谱(SEC-HPLC)

评论(0)