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产品规格
物种
Human分子别名
BRAF1,RAFB1Accession
P15056表达序列
E433-R726表达宿主
Baculovirus-InsectCells分子量
60.3 kDa
纯度
> 35% by SDS-PAGE内毒素含量
<1EU/μg标记
Unconjugated标签
GST Tag性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 20 mM Reduced glutathione, 10% glycerol, 0.05% Brij35, pH7.5储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The BRAF activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the BRAF protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
电泳
M MW Standard
1 1 μg BRAF
1 3 μg BARF
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