所有图片（1/3）
分享产品介绍 评论(0)
产品规格
物种
Human分子别名
EGFR(d746-750/T790M/C797S)Accession
P00533-1表达序列
R669-A1210(end), d746-750, T790M, C797S表达宿主
Baculovirus-InsectCells分子量
87.2 kDa
纯度
> 90% by SDS-PAGE标签
GST Tag性状
Liquid缓冲体系
50 mM Hepes, 150 mM NaCl, 1 mM DTT, 10% Glycerol, 0.05% Brij35, pH7.5
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt.
And avoid repeated freeze-thaws cycles.
生物活性
EGFR[d746-750/T790M/C797S] titration and inhibitor IC50 determination
The EGFR[d746-750/T790M/C797S] activity was detected using HTRF technology. The reaction was performed by incubating the EGFR[d746-750/T790M/C797S] protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
电泳
M MW Standard
1 1 μg EGFR[d746-750/T790M/C797S]
2 3 μg EGFR[d746-750/T790M/C797S]
评论(0)