分享产品介绍 评论(0)
物种
Human分子别名
HTK,MYK1,TYRO11,EPHB4Accession
P54760-1表达序列
K577-Y987(end)
KHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFELVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGTPGGTGGPAPQY
表达宿主
Baculovirus-InsectCells分子量
70386.0纯度
>95% by SDS-PAGE内毒素含量
<1EU/μg活性
HTRF_Function标记
Unconjugated性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 20mM Reduced Glu, 1mM DTT, 0.05% Brij-35, 0.1mM PMSF，10% glycerol, pH 7.5储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
EPHB4 titration in HTRF assay
The EPHB4 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the EPHB4 protein , ATP and substrate at 25℃ for 60 min, then reading 665/620 ratio with BMG.
电泳
体积排阻色谱(SEC-HPLC)
评论(0)