分享产品介绍 评论(0)
物种
HumanAccession
P54762-1表达序列
K578-A984(end)
KLQHYSTGRGSPGMKIYIDPFTYEDPNEAVREFAKEIDVSFVKIEEVIGAGEFGEVYKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMIITEFMENGALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLAEMNYVHRDLAARNILVNSNLVCKVSDFGLSRYLQDDTSDPTYTSSLGGKIPVRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPMDCPAALHQLMLDCWQKDRNSRPRFAEIVNTLDKMIRNPASLKTVATITAVPSQPLLDRSIPDFTAFTTVDDWLSAIKMVQYRDSFLTAGFTSLQLVTQMTSEDLLRIGITLAGHQKKILNSIHSMRVQISQSPTAMA
表达宿主
Baculovirus-InsectCells分子量
72.6 kDa纯度
>90% by SDS-PAGE内毒素含量
<1EU/μg活性
HTRF_Function标记
Unconjugated标签
GST Tag性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 20mM Reduced Glu, 1mM DTT, 0.05% Brij-35, 10% glycerol, pH 7.5储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
EPHB1 titration in HTRF assay
The EPHB1 activity was detected using HTRF technology. The reaction was performed by incubating the EPHB1 protein , ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
电泳
体积排阻色谱(SEC-HPLC)
评论(0)