产品介绍 评论(0)
产品规格
物种
HumanAccession
P29320-1表达序列
K579-V983(end)表达宿主
Baculovirus-InsectCells分子量
71.9 kDa纯度
>95% by SDS-PAGE内毒素含量
<1EU/μg活性
HTRF_Function标记
Unconjugated性状
Liquid缓冲体系
50mM Tris, 150mM NaCl, 1mM DTT, 10% Glycerol, 0.05% Briji-35, pH7.5储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
EPHA3 titration in HTRF assay
The EPHA3 activity was detected using homogeneous time-resolved fluorescence technology. The reaction was performed by incubating the EPHA3 protein , ATP and substrate at 25℃ for 50 min, adding the TK-mix at 25℃ for 60 min, then reading ratio 665/620 nm with BMG.
电泳
体积排阻色谱(SEC-HPLC)











评论(0)