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物种
HumanAccession
P29317-1表达序列
E572-I976(end)
EDVYFSKSEQLKPLKTYVDPHTYEDPNQAVLKFTTEIHPSCVTRQKVIGAGEFGEVYKGMLKTSSGKKEVPVAIKTLKAGYTEKQRVDFLGEAGIMGQFSHHNIIRLEGVISKYKPMMIITEYMENGALDKFLREKDGEFSVLQLVGMLRGIAAGMKYLANMNYVHRDLAARNILVNSNLVCKVSDFGLSRVLEDDPEATYTTSGGKIPIRWTAPEAISYRKFTSASDVWSFGIVMWEVMTYGERPYWELSNHEVMKAINDGFRLPTPMDCPSAIYQLMMQCWQQERARRPKFADIVSILDKLIRAPDSLKTLADFDPRVSIRLPSTSGSEGVPFRTVSEWLESIKMQQYTEHFMAAGYTAIEKVVQMTNDDIKRIGVRLPGHQKRIAYSLLGLKDQVNTVGIPI
表达宿主
Baculovirus-InsectCells分子量
72.5 kDa纯度
>90% by SDS-PAGE内毒素含量
<1EU/μg活性
HTRF_Function标记
Unconjugated性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 20mM Reduced Glu, 10% glycerol, 1mM DTT, pH 8.0储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
EPHA2 titration in HTRF assay
The EPHA2 activity was detected using homogeneous time-resolved fluorescence technology. The reaction was performed by incubating the EPHA2 protein , ATP and substrate at 25℃ for 50 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 nm with BMG.
电泳
体积排阻色谱(SEC-HPLC)
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