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产品规格
物种
HumanAccession
P24941-1, P24864-1表达序列
CDK2:M1-L298(end);CycE1:M1-A410(end)表达宿主
Baculovirus-InsectCells分子量
CDK2: 60.5 kDa; CycE1: 73.7 kDa
纯度
>95% by SDS-PAGE内毒素含量
<1EU/μg活性
ADP-Glo_Function标记
Unconjugated标签
GST Tag性状
Liquid缓冲体系
20mM Tris, 150mM Nacl,1mM DTT, 10% glycerol, pH7.4储存条件
-80℃
生物活性
CDK2/CycE1 titration in ADP-Glo assay
The CDK2-CycE1 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the CDK2-CycE1 protein , ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
电泳
体积排阻色谱(SEC-HPLC)











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