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产品规格
物种
HumanAccession
Q9NYV4-1, O75909-3表达序列
CDK12: Q696-S1082;Cyclin K: M1-S300表达宿主
Baculovirus-InsectCells分子量
CDK12: 61.7 kDa; Cyclin K: 48.1 kDa纯度
>85% by SDS-PAGE内毒素含量
<1EU/μg活性
ADP-Glo_Function标记
Unconjugated性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 20 mM reduced glutathione , 1 mM DTT, 0.05% Brij35, 10% glycerol, pH 7.5储存条件
-80℃
生物活性
CDK12/Cyclin K titration in ADP-Glo assay
The CDK12/Cyclin K activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the CDK12/Cyclin K protein , ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
电泳
体积排阻色谱(SEC-HPLC)











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