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产品规格
物种
HumanAccession
P06493-1, P24864-1表达序列
CDK1 :M1-M297(end);CycE1:M1-A410(end)表达宿主
Baculovirus-InsectCells分子量
CDK1: 60.7 kDa; CycE1: 73.7 kDa纯度
>90% by SDS-PAGE内毒素含量
<1EU/μg活性
ADP-Glo_Function标记
Unconjugated性状
Liquid缓冲体系
50mM Hepes, 150mM Nacl, 20mM Reduced Glu, 1mM DTT, 10% glycerol, pH7.5储存条件
-80℃
生物活性
CDK1/CycE1 titration in ADP-Glo assay
The CDK1-CycE1 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the CDK1-CycE1 protein , ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
电泳
体积排阻色谱(SEC-HPLC)











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