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产品规格
物种
Human分子别名
BTK[V416L]Accession
Q06187-1表达序列
A2-S659(end)表达宿主
Baculovirus-InsectCells分子量
101.9 kDa纯度
>95% by SDS-PAGE内毒素含量
<1EU/μg活性
HTRF_Function标记
Unconjugated性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.1 mM PMSF, 10% Glycerol, 0.05% Brij35, pH 7.5储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The BTK[V416L] activity was detected using HTRF technology. The reaction was performed by incubating the BTK[V416L] protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
电泳
体积排阻色谱(SEC-HPLC)
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