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产品规格
物种
Human分子别名
AGMX1,ATK,BPKAccession
Q06187-1表达序列
A2-S659(end) N-terminal表达宿主
Baculovirus-InsectCells分子量
101.9 kDa
纯度
＞95%, by SDS-PAGE under reducing conditions
＞95%，as determined by HPLC
活性
HTRF_Function标记
Unconjugated标签
GST Tag性状
Liquid缓冲体系
20 mM Hepes, 300 mM NaCl, 1 mM DTT, 0.1 mM PMSF, 10% Glycerol, 0.05% Brij-35, pH7.5储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The BTK activity was detected using HTRF technology. The reaction was performed by incubating the BTK protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
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