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产品规格
物种
HumanAccession
P36896-1表达序列
N150-I505表达宿主
Baculovirus-InsectCells分子量
67.4 kDa纯度
>95% by SDS-PAGE内毒素含量
<1EU/μg活性
ADP-Glo_Function标记
Unconjugated标签
GST Tag性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 20 mM reduced glutathione, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH 7.5储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The ALK4 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the ALK4 protein, ATP and substrate at 25℃ for 60 min, and adding ADP-Glo reagent at 25℃ for 40 min, then reading RLU with BMG.
电泳
体积排阻色谱(SEC-HPLC)
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