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产品规格
物种
Human分子别名
PRKCQ,PRKCT,MGC126514,MGC141919,nPKC-theta,PKCθ protein kinase c thetaAccession
O75417-1表达序列
S1819-V2590(end)
表达宿主
Baculovirus-InsectCells分子量
88602.0
纯度
>95% by SDS-PAGE内毒素含量
<1EU/μg活性
HTRF_Function/ABS_Function标记
Unconjugated标签
His Tag性状
Liquid缓冲体系
20 mM Tris, 500 mM NaCl, 2 mM B-ME, 5% glycerol, pH8.5储存条件
-80℃
生物活性
The POLQ-C activity was detected using TR-FRET technology. The reaction was performed by incubating the POLQ-C protein, fluorescence-labeled dsDNA and dNTP (dCTP, dGTP, dTTP) at 25℃ for 40 min, then reading ratio 520/485 fluorescence signal with BMG.
电泳
M MW Standard
1 1 μg POLQ-C
2 3 μg POLQ-C
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