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产品规格
物种
Human分子别名
Protein mono-ADP-ribosyltransferase PARP11,ADP-ribosyltransferase diphtheria toxin-like 11,Poly [ADP-ribose] polymerase 11,C12orf6Accession
Q9NR21-4表达序列
M8-H338(end)表达宿主
Baculovirus-InsectCells分子量
64900
纯度
＞90% by SDS-PAGE内毒素含量
<1EU/μg活性
ELISA_Function标记
Unconjugated标签
GST Tag, His Tag性状
Lyophilized Powder缓冲体系
20 mM Hepes, 300 mM NaCl, pH7.5, 1 mM DTT, 10% glycerol储存条件
-80℃
生物活性
The PARP11 activity was assayed with ELISA. The reaction was performed by: 1). Histone protein was coated on a 96-well plate. 2).A biotinylated NAD+ mix is incubated with varying different concentration of the PARP11 enzyme and an activated DNA. 3).The plate was treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence. Finally, measured the signal using a chemiluminescence reader.
电泳
M MW Standard
1 1 μg PARP11
2 3 μg PARP11
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