PARP2, GST Tag Protein
Poly [ADP-ribose] polymerase 2,PARP-2,hPARP-2,ADP-ribosyltransferase diphtheria toxin-like 2,ARTD2,DNA ADP-ribosyltransferase PARP2,NAD(+) ADP-ribosyltransferase 2,ADPRT-2,Poly[ADP-ribose] synthase 2,pADPRT-2,Protein poly-ADP-ribosyltransferase
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物种
Human分子别名
Poly [ADP-ribose] polymerase 2,PARP-2,hPARP-2,ADP-ribosyltransferase diphtheria toxin-like 2,ARTD2,DNA ADP-ribosyltransferase PARP2,NAD(+) ADP-ribosyltransferase 2,ADPRT-2,Poly[ADP-ribose] synthase 2,pADPRT-2,Protein poly-ADP-ribosyltransferaseAccession
Q9UGN5表达序列
A2-W583(end)表达宿主
Baculovirus-InsectCells分子量
92.4 kDa
纯度
>85% by SDS-PAGE内毒素含量
<1EU/μg活性
HTRF_binding/SPR/ELISA_Function标记
Unconjugated标签
GST Tag性状
Liquid缓冲体系
20 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The PARP2 activity was assayed with ELISA. The reaction was performed by: 1). Histone protein was coated on a 96-well plate. 2).A biotinylated NAD+ mix is incubated with varying different concentration of the PARP2 enzyme and an activated DNA. 3).The plate was treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence. Finally, measured the signal using a chemiluminescence reader.
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