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产品规格
物种
Human分子别名
poly (ADP-ribose) polymerase 1,poly(ADP-ribosyl)transferase,ADPRT,PARP1,PARP-1Accession
P09874表达序列
A2-W1014(end)表达宿主
Baculovirus-InsectCells分子量
115077.0
纯度
>85% by SDS-PAGE内毒素含量
<1EU/μg活性
HTRF_binding/SPR/ELISA_Function标记
Unconjugated标签
His Tag性状
Liquid缓冲体系
20 mM Hepes, 300 mM NaCl, 1mM DTT, pH7.0储存条件
-80℃
生物活性
The PARP1 activity was assayed with ELISA. The reaction was performed by: 1). Histone protein was coated on a 96-well plate. 2).A biotinylated NAD+ mix is incubated with varying different concentration of the PARP1 enzyme and an activated DNA. 3).The plate was treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence. Finally, measured the signal using a chemiluminescence reader.
电泳
M MW Standard
1 1 μg PARP1
2 3 μg PARP1
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