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Modified Trypsin, Mass Spectrometry Grade

Trypsin(pig),porcine pancreas,Trypsin,Mass Spectrometry Grade

价格 ￥1,700.00 （ 1-2周）
货号 UA070137
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 分子别名

    Trypsin(pig); porcine pancreas; Trypsin，Mass Spectrometry Grade

  • 表达宿主

    E.coli

  • 分子量

    24±2 kDa (Reducing)

  • 纯度

    ＞95% by RP- HPLC

  • 活性

    ≥6000 USP/mg

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    2 mM HCl, 2 mM Ca2+

  • 溶解方法

    Modified Trypsin, Mass Spectrometry Grade should be reconstituted by the addition of 20~200 μL of 2 mM HCl or 50mM acetic acid.

  • 储存条件

    Store at -25 ~ -15℃ for 2 years

  • 文献引用

    Olsen, J. V. Trypsin Cleaves Exclusively C-terminal to Arginine and Lysine Residues[J]. Molecular & Cellular Proteomics, 2004, 3(6):608-614.
    Chi W J. Medium Optimization and Application of Affinity Column Chromatography for Trypsin Production from Recombinant Streptomyces griseus[J]. Journal of Microbiology & Biotechnology, 2009, 19(10):1191-1196.

背景介绍

  • Modified Trypsin, Mass Spectrometry Grade is produced through a multi-step process: the wild-type trypsin sequence is engineered to minimize autolysis, the modified enzyme is then expressed recombinantly to eliminate contaminating proteases, and finally subjected to reductive methylation to further enhance stability. The resulting product exhibits high purity and exceptional stability, making it ideal for a broad range of laboratory proteomics applications, including protein/peptide sequencing and identification, as well as HPLC peptide mapping.

操作步骤

  • 1 Dissolve Modified Trypsin by adding 20 to 200 μL of 2 mM HCL or 50 mM acetic acid, and prepare 2*Reaction buffer (100 mM Tris-HCl, 10 mM CaCl2, pH 8.0 @ 25°C)

    2 Set-up a typical reaction as follows

    1）Add the following components in sequence

    Components

    Volume

    Substrate protein

    5 μL (about 2 ug)

    ddH2O

    4.5 μL

    2*Reaction buffer

    10 μL

    100 ng/μL UA070137

    0.5 μL


    2）Incubate at 37°C for 2~4 h or overnight

注意事项

  • 1. Substrate must be in phosphate-free buffer to prevent calcium precipitation with both reconstituted enzyme and enzyme buffer.

    2. Storage buffer:2 mM HCl or 50mM acetic acid

酶活定义
  • In a 3.2 mL reaction system using BAEE as substrate, the UV absorption at 253 nm increased by 0.003 per min using cuvette with 1 cm optical path, which was defined as 1 USP trypsin activity unit

  • 生物活性

  • 电泳

    • 2μg Modified Trypsin, Mass Spectrometry Grade
      R: reducing condition

  • 反相高效液相色谱(RP-HPLC)

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