热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 酶制剂 抗体及糖基化切割

所有图片（1/4）

Spe B

Streptopain,Exotoxin type B,Group A streptococcal cysteine protease (Streptococcal cysteine proteinase),SPE B,Streptococcus peptidase A (SPP)

价格 ￥1,200.00 （ 供应商现货 ： 3-5个工作日）
货号 UA070131
规格
数量
收藏 分享
产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Streptococcus pyogenes

  • 分子别名

    Streptopain, Exotoxin type B, Group A streptococcal cysteine protease (Streptococcal cysteine proteinase), SPE B, Streptococcus peptidase A (SPP)

  • 表达宿主

    E.coli

  • 分子量

    29kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE&HPLC

  • 活性

    40U/µL

  • 标签

    His Tag

  • 性状

    Liquid

  • 缓冲体系

    20mM Tris-HCl, 0.5M NaCl, 50% Glycerol (pH 7.0)

  • 储存条件

    Store at -25 ~ -15℃ for 2 years

  • 文献引用

    [1] Musser J M , Stockbauer K , Kapur V ,et al.Substitution of cysteine 192 in a highly conserved Streptococcus pyogenes extracellular cysteine protease (interleukin 1beta convertase) alters proteolytic activity and ablates zymogen processing.[J].Infection & Immunity, 1996, 64(6):1913.
    [2] Persson H , Vindebro R , Von Pawel-Rammingen U .The streptococcal cysteine protease SpeB is not a natural immunoglobulin-cleaving enzyme.[J].Infection & Immunity, 2013, 81(6).

背景介绍

  • SpeB is a unique recombinant cysteine protease that can cleave the hinge regions of various species and subclasses of antibodies, thereby generating a homogeneous pool of Fab fragments. This enzyme requires reducing conditions when acting on IgG. SpeB can digest human, mouse, rat, goat and sheep IgG, thereby producing Fab and Fc fragments. The main digestion site of human IgG1 is KTHT/CPPCPAPE.

产品组分

  • UA070131: 40U/µL SpeB in 20mM Tris-HCl, 0.5M NaCl, 50% Glycerol (pH 7.0)
    5*Reaction Buffer: 5*PBS, 25mM DTT (pH 7.4)

操作步骤

  • 1.Take up to 10µg of human IgG1, adjust to 39µl with purified water;
    2.Add 10µl of 5*Reaction Buffer;
    3.Add 1µl SpeB to a total volume of 50µl, mix gently;
    4. React for 1 hour at 37°C.

注意事项

  • 1. For different antibodies sample, the optimal enzyme concentration and reaction time need to be mapped experimentally;

    2. Higher enzyme concentrations increase the digestion efficiency of individual antibodies and need to be optimized accordingly.

酶活定义
  • One unit digests ≥ 95% of 1 µg human IgG1 when incubated in PBS with 5 mM DTT or TCEP, pH 7.4 at 37°C for 1 hour.

  • 生物活性

    • Results of 5 μg human IgG1 digestion separated under different quantity of SpeB, The reaction was incubated in PBS with 5 mM DTT, pH 7.4 at 37°C for 1 hour.
      M Marker
      Lane 1 Negative Control(only UA070131)
      Lane 2 Negative Control(only human IgG1)
      Lane 3 UA070131-SpeB 2.5 U
      Lane 4 UA070131-SpeB 5 U
      Lane 5 UA070131-SpeB 10 U

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • 97.9%

  • 反相高效液相色谱(RP-HPLC)

    • 98%

评论(0)