热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 酶制剂 蛋白酶

所有图片（1/2）

Kex2

Kexin,Protease KEX2

价格 ￥750.00 （ 1-2周）
货号 UA070082
规格
数量
收藏 分享
产品介绍

产品介绍 评论(0)

产品规格

  • 分子别名

    Kexin,Protease KEX2

  • 表达宿主

    Pichia pastoris

  • 分子量

    75 kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 活性

    ≥10U/mg

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    20mM NaAC-HAC，2mM Ca2+，ph5.2 @ 25°C

  • 储存条件

    Store at -25 ~ -15℃ for 2 years

  • 文献引用

    1. Kasai N .Identification of the fourth member of the mammalian endoprotease family homologous to the yeast Kex2 protease[J].Journal of Biological Chemistry, 1992, 267(1):45-63.
    2. Matsuo T H . Characterization of KEX2-encoded endopeptidase from yeast Saccharomyces, cerevisiae[J].Biochemical and Biophysical Research Communications, 1989.

背景介绍

  • Kex2 protease, a precursor processing protease derived from yeast, is a calcium-dependent serine protease that specifically recognizes and cleaves the carboxy-terminal peptide bond of the bi-alkaline amino acids Arg-Arg, Lys-Arg, and Pro-Arg. Unlike trypsin, Kex2 does not recognize and cleave the carboxy-terminal peptide bond of a single basic amino acid, arginine or lysine. In yeast, Kex2 protease is responsible for the processing of killer toxin and α-factor precursors. Kex2 protease activity is not inhibited by conventional serine protease inhibitors such as peptidyl peptidase, PMSF and TPCK. The recombinant Kex2 protease is produced by expression of Saccharomyces cerevisiae and has the same enzyme specificity as the natural Saccharomyces cerevisiae Kex2 enzyme. The optimal pH for action is pH 9.0, and the pH for stable storage is pH 5.0-6.0.

产品组分

  • Kex2 lyophilized
    Dissolved buffer: 20mmol/L NaAc-HAc, 2mmol/L Ca2+, pH 5.0~5.5
    Reaction buffer:50 mM Tris-HCl、2mM Ca2+、(pH 8.0 @ 25°C)

操作步骤

  • Kex2 should be reconstituted by the addition of 20–100 μl of Dissolved buffer. Typical reaction conditions are as follows:
    Combine 500 ug of sample with reaction buffer *（Recommended 50 mM Tris-HCl、2mM Ca2+、(pH 8.0 @ 25°C))）
    Add 5μL(5μg)of Kex2，Incubate at 25°C for 3 hours
    Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate Recommended ratio: protease: fusion protein amount ratio 1:20~1:1000

注意事项

  • 1.Reaction buffer: pH 7.0-9.0, 50mM Tris-HCl, 2mM Ca2+;

    2.If the lyophilized powder cannot be used immediately after dissolution, it is recommended to dissolve the lyophilized powder with 20mM (pH5.2) NaAc-HAc, 2mM Ca2+ buffer;

    3.The final concentration of the enzyme after dissolution will be stored at -20℃ for storage after dispensing as needed;

    4.The optimal reaction pH for Kex2 is pH 9.0, and the optimal pH for storage is 5.0-6.0.

酶活定义
  • One unit refers to the amount of enzyme required to catalyze the conversion of 1 µmol of BOC-Gln-Arg-Arg-Arg-PNA to the product per minute at 25°C, pH 8.0.

  • 生物活性

    • ≥10U/mg

      Take 2.89ml of assay buffer, 100µL of substrate solution, add 10µL of enzyme solution, mix well and time immediately, keep the temperature in the colorimetric cell at 25°C, read the absorbance at 405nm at 20s intervals for 3-4min.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

评论(0)