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物种
Bovine分子别名
Carboxypeptidase B表达宿主
PichiaPastoris分子量
33 kD
纯度
＞95% by SDS-PAGE & HPLC活性
120 U/mg标签
His Tag性状
Lyophilized Powder缓冲体系
20mM Tris-HCl 、100mM NaCl
溶解方法
Carboxypeptidase B lyophilized powder was dissolved in sterile water or 25mM Tris-HCl (pH7.6) to make the concentration of enzyme solution 1-10mg /ml.
储存条件
·24 month, 2 to 8 °C under sterile conditions
. After dissolution, store at -20℃ and avoid repeated freeze-thaw cycles.文献引用
[1] Akparov V, Sokolenko N, Timofeev V, Kuranova I. Structure of the complex of carboxypeptidase B and N-sulfamoyl-L-arginine. Acta Crystallogr F Struct Biol Commun. 2015 Oct;71(Pt 10):1335-40.
[2] James W. Brodrick, Michael C. Geokas, Corey Largman, Human carboxypeptidase B. II. Purification of the enzyme from pancreatic tissue and comparison with the enzymes present in pancreatic secretion, Biochimica et Biophysica Acta (BBA) - Enzymology, 1976, 468-481.
Carboxypeptidase B, also known as peptidyl-L-lysine (L-arginine) hydrolase, contains one Zn atom per molecule and can selectively hydrolyze arginine and lysine at the carboxyl end of proteins or polypeptides. CPB can be used in the production of recombinant insulin and its analogues, and the quality detection of recombinant antibodies. and also used in the production of other recombinant polypeptides.
20mM Tris-HCl 、100mM NaCl、Mannitol、Carboxypeptidase B
1. For different protein samples, it is necessary to explore the optimal enzyme concentration and reaction time.
2. Reaction at 25℃~37℃, the reaction time can be appropriately extended when digestion at 25℃.
- The amount of enzyme that catalyzes the hydrolysis of 1 μmol of maroyl-L-arginine in 1 min is one enzyme activity unit of carboxypeptidase B.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
反相高效液相色谱(RP-HPLC)
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