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产品中心 酶制剂 抗体及糖基化切割

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糖苷内切酶 S2

Endo-beta-N-acetylglucosaminidase EndoS2、Endoglycosidase S2、EndoS2

价格 ￥2,880.00 （ 供应商现货 ： 3-5个工作日）
货号 UA070055
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Streptococcus pyogenes

  • 分子别名

    Endo-beta-N-acetylglucosaminidase EndoS2、Endoglycosidase S2、EndoS2

  • 表达宿主

    E.coli

  • 分子量

    93 kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 活性

    200U/ul

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Liquid

  • 缓冲体系

    20 mM Tris-HCl、50 mM NaCl、pH 7.5 @ 25°C

  • 储存条件

    Store at -25 ~ -15℃ for 2 years

  • 文献引用

    [1] Sjögren Jonathan, et al. "EndoS and EndoS2 hydrolyze Fc-glycans on therapeutic antibodies with different glycoform selectivity and can be used for rapid quantification of high-mannose glycans." Glycobiology 10:1053-1063.
    [2] Jonathan Sjögren, et al. "EndoS2 is a unique and conserved enzyme of serotype M49 group A Streptococcus that hydrolyses N-linked glycans on IgG and α1-acid glycoprotein. " Biochemical Journal 455.Pt 1(2013).

背景介绍

  • Endo-S2 is an endo-n-acetylglucosaminase derived from streptococcus pyogenes that excises the glycosidic bond between the two innermost n-acetylglucosamines of the glycoprotein n-linking sugar chain. It is effective for high mannose type, hybrid type and double antenna complex type. Can be made for all human IgG, as well as mice, rats, monkey, goat, sheep, cattle and horse IgG antibody glycosyl.

产品组分

  • Storage Solution : 200U/ul Endo S2 、20 mM Tris-HCl, 50 mM NaCl. (pH 7.5 @ 25°C)

    10*Reaction Buffer： 500 mM sodium acetate (pH 6.0 @ 25°C)

操作步骤

  • 1. Combine 100 µg of native IgG, 1 µl of 10*Reaction Buffer and H20 (if necessary) to make a 10 µl total reaction volume.

    2. Add 1 µl EndoS2.

    3. Incubate reaction at 37°C for 1 hour

注意事项

  • Please avoid repeated freeze-thaw cycles.

酶活定义
  • One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 5 μg of native mouse monoclonal IgG in 1 hour at 37°C in a total reaction volume of 10 µl.

  • 生物活性

    • The experimental design added 20 U, 10 U, 5 U, 2.5 U, 1U and 0.5 U enzymes into the 20 ul reaction system to investigate the effect of Endo S2 on the digestion of human IgG.
      M marker
      Lan1 human IgG 5μg
      Lan2 human IgG 5μg+20 U Endo S2
      Lan3 human IgG 5μg+10 U Endo S2
      Lan4 human IgG 5μg+5 U Endo S2
      Lan5 human IgG 5μg+2.5 U Endo S2
      Lan6 human IgG 5μg+1 U Endo S2
      Lan7 human IgG 5μg+0.5 U Endo S2

  • 电泳

    • 1μg (R: reducing condition, N: non-reducing condition).

  • 反相高效液相色谱(RP-HPLC)

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