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Proteinase K(Tag free)

Proteinase K

价格 ￥500.00 （ 1-2周）
货号 UA070054
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 分子别名

    Proteinase K

  • 表达宿主

    PichiaPastoris

  • 分子量

    72 kD

  • 纯度

    ＞95% by SDS-PAGE

  • 活性

    400 units/ml（10mg/ml）

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    20 mM Tris-HCl、1 mM CaCl2、50% Glycerol、pH 7.4 @ 25°C

  • 储存条件

    Store at -25 ~ -15℃ for 1 years

  • 文献引用

    [1] Kushnirov VV, Dergalev AA, Alexandrov AI. Proteinase K resistant cores of prions and amyloids. Prion. 2020 Dec;14(1):11-19.
    [2] Xu C , Battig A , Schartel B ,et al.Investigation of the Thermal Stability of Proteinase K for the Melt Processing of Poly(l-lactide)[J].Biomacromolecules, 2022, 23(11):4841-4850.

背景介绍

  • Proteinase K is a powerful proteolytic enzyme with high specific activity and is a key reagent for DNA extraction. The enzyme is active in a wide pH range (4~12.5) and at high temperature (50~70°C), and is used for the isolation of plasmid or genomic DNA and RNA. In DNA extraction, the main function is to enzymatically dissociate the histones bound to nucleic acids, so that the DNA is free in the solution, and then extracted by different methods to remove impurities and collect the DNA. chelating agents such as EDTA or descaling agents such as SDS can not inactivate the enzyme.

产品组分

  • Storage Solution:20 mM Tris-HCl、1mM CaCl2、50% glycerol (pH 7.4 @ 25°C)

操作步骤

  • Add the specified amount of Proteinase K storage solution according to the nucleic acid extraction instructions. Commonly used working concentrations are 50-100 µg/mL

注意事项

  • Please avoid repeated freeze-thaw cycles.

酶活定义
  • One unit is defined as amount of enzyme required to hydrolyze casein to produce 1µmoL L-tyrosine per minute under defined conditions.

  • 电泳

    • M: marker
      Lane 1: 2μg PK is reduced and thermally denatured;
      Lane 2: 2μg PK under reducing condition;
      Lane 3: 2μg PK is non-reduced and thermally denatured;
      Lanes 4: 2μg PK under non-reducing condition;

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