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分子别名
Ribonuclease R、RNase R表达宿主
E.coli分子量
94 kD
纯度
＞95% by SDS-PAGE活性
20U/μl标记
Unconjugated标签
His Tag性状
Liquid缓冲体系
50mM Tris-HCl, 200mM NaCl, 1mM DTT, 0.1mM EDTA, 50% (v/v) Glycerol, 0.1% (w/v) Triton X-100 (pH7.5 @25℃)
储存条件
Store at -25 ~ -15℃ for 1 years
文献引用
[1] Cheng, Z.-F. Purification and Characterization of the Escherichia coli Exoribonuclease RNase R COMPARISON WITH RNase II[J]. Journal of Biological Chemistry, 2002, 277(24):21624.
[2] Cheng Z F, Deutscher M P. An Important Role for RNase R in mRNA Decay[J].Molecular Cell, 2005, 17(2):313-318.
Ribonuclease R (RNase R) is a Mg2+ dependent 3'→5' exonuclease derived from Escherichia coli. RNase R can digest all linear RNA. However, it cannot digest ring-shaped RNA, lasso structure or double-stranded RNA molecules with 3 'end protruding ends less than 7 nucleotides, tRNA and 5SRNA with complex secondary structure. RNase R is commonly used in gene expression and variable shear studies and can digest linear RNA to enrich circular RNA or lasso structured RNA. This product does not contain DNase, other RNA endonuclease and exonuclease activities.
Storage Solution: 20U/μl RNase R, 50mM Tris-HCl, 200mM NaCl, 1mM DTT, 0.1mM EDTA, 50% (v/v) Glycerol, 0.1% (w/v) Triton X-100 (pH7.5 @25℃) 10*Reaction Buffer: 200mM Tris-HCl, 1M KCl, 1mM MgCl2 (pH8.0 @25℃)
1. The activity of RNase R requires 0.1-1.0 mM Mg2+;
2. With the increase of substrate RNA, digestion time and enzyme amount can be appropriately prolonged
- One unit converts 1μg of poly-r(A) into acid-soluble nucleotides in 10 minutes at 37℃ in 20mM Tris-HCl (pH8.0), 100mM KCl and 0.1mM MgCl2
生物活性
In the 20µl reaction system, the mixture of linear and circular RNA was used as substrate, nuclease-free water was added to the control group, RNase R was added to the experimental group, the reaction was performed at 37℃ for 30min. The reaction was analyzed by 1% agarose gel electrophoresis.
M: DNA marker
Lane 1 negative control
Lane 2 Experimental result
电泳
1μg (R: reducing condition, N: non-reducing condition).
引用文献(1)
- CircPIK3C2A dynamically orchestrates autophagy and apoptosis to enhance antibacterial immunity in yellow catfish (Pelteobagrus fulvidraco)
L Zhou, S Xiao, Y Shi, B Han, S Yin, X Ning - Aquaculture, 2025 - Elsevier
Aquaculture. 2025 Sep 30 .
影响因子： 3.9
货号：UA070049产品名称：RNase R
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